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Assembly of root-associated bacteria communities: Interactions between abiotic and biotic factors, 2015


Dean, S. L., Farrer, E. C., Porras-Alfaro, A., Suding, K. N., Sinsabaugh, R. L.


Environmental Microbiology Reports 7 (1) :102-110
DOI: 10.1111/1758-2229.12194

Abstract

Nitrogen (N) deposition in many areas of the world is over an order of magnitude greater than it would be in absence of human activity. We ask how abiotic (N) and biotic (plant host and neighborhood) effects interact to influence root-associated bacterial (RAB) community assembly. Using 454 pyrosequencing, we examined RAB communities from two dominant alpine tundra plants, Geum rossii and Deschampsia cespitosa, under control, N addition and D. cespitosa removal treatments, implemented in a factorial design. We hypothesized that host would have the strongest effect on RAB assembly, followed by N, then neighbor effects.

The most dominant phyla were Proteobacte- ria (mostly Gammaproteobacteria), Actinobacteria, Bacteroidetes and Acidobacteria. We found RAB communities were host specific, with only 17% overlap in operational taxonomic units. Host effects on composition were over twice as strong as N effects. D. cespitosa RAB diversity declined with N, while G. rossii RAB did not. D. cespitosa removal did not influence G. rossii RAB community composition, but G. rossii RAB diversity declined with N only when D. cespitosa was absent.

We conclude that RAB of both hosts are sensitive to N enrichment, and RAB response to N is influenced by host identity and plant neighborhood. 

Associated Niwot LTER Researchers:

Related Field Location(s):

Alpine zone

Related Data by Discipline(s):

Microbial ecology

Citation

Dean, S. L., Farrer, E. C., Porras-Alfaro, A., Suding, K. N., Sinsabaugh, R. L., (2015) Assembly of root-associated bacteria communities: Interactions between abiotic and biotic factors. Environmental Microbiology Reports 7 (1) :102-110 , DOI: 10.1111/1758-2229.12194

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CU Boulder

This material is based upon work supported by the National Science Foundation under Cooperative Agreement #DEB-1637686. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necesarily reflect the views of the National Science Foundation.

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